› 2cvx

X-ray diffraction
2.2Å resolution

Structures of Yeast Ribonucleotide Reductase I

Released:
DOI: 10.2210/pdb2cvx/pdb
PDB entry 2cvx coloured by chain, front view.
PDB entry 2cvx coloured by chain, side view.
PDB entry 2cvx coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
Xu H, Faber C, Uchiki T, Fairman JW, Racca J, Dealwis C
Proc Natl Acad Sci U S A 103 4022-7 (2006)
PMID: 16537479

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large chain 1 Chain: A
Molecule details ›
Chain: A
Length: 888 amino acids
Theoretical weight: 99.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21524 (Residues: 1-888; Coverage: 100%)
Gene names: CRT7, RIR1, RNR1, SDS12, YER070W
Sequence domains:
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand DGT 1 x DGT
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 107.687Å b: 117.277Å c: 65.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.21 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

DNA building blocks: keeping control of manufacture.
Hofer et al. (2012)
7 more

5 mentions without citation

The origin and evolution of ribonucleotide reduction.
Lundin et al. (2015)
4 more