› 2cwa

X-ray diffraction
1.96Å resolution

Crystal Structure Of The Single-stranded DNA Binding Protein From Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb2cwa/pdb
PDB entry 2cwa coloured by chain, front view.
PDB entry 2cwa coloured by chain, side view.
PDB entry 2cwa coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Satoh S, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.11 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLP9 (Residues: 1-263; Coverage: 100%)
Gene names: TTHA0244, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand NO3 3 x NO3
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P6522
Unit cell:
a: 91.56Å b: 91.56Å c: 132.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.242 0.282
Expression system: Escherichia coli

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