› 2cwl

X-ray diffraction
1.65Å resolution

Crystal structure of manganese-free form of pseudocatalase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2cwl/pdb
PDB entry 2cwl coloured by chain, front view.
PDB entry 2cwl coloured by chain, side view.
PDB entry 2cwl coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Ebihara A, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese-containing pseudocatalase Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.37 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SM21 (Residues: 1-302; Coverage: 100%)
Gene name: TTHA0122
Sequence domains: Manganese containing catalase
Structure domains: Ferritin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P213
Unit cell:
a: 131.36Å b: 131.36Å c: 131.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.181
Expression system: Escherichia coli BL21(DE3)

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