› 2cww

X-ray diffraction
2.6Å resolution

Crystal structure of Thermus thermophilus TTHA1280, a putative SAM-dependent RNA methyltransferase, in complex with S-adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb2cww/pdb
PDB entry 2cww coloured by chain, front view.
PDB entry 2cww coloured by chain, side view.
PDB entry 2cww coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine.
Pioszak AA, Murayama K, Nakagawa N, Ebihara A, Kuramitsu S, Shirouzu M, Yokoyama S
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 867-74 (2005)
PMID: 16511182

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 43.07 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIT4 (Residues: 1-382; Coverage: 100%)
Gene name: TTHA1280
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SAH 2 x SAH
Ligand ACY 1 x ACY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 137.805Å b: 46.065Å c: 134.127Å
α: 90° β: 109.93° γ: 90°
R-values:
R R work R free
0.302 0.258 0.297
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein universe containing a PUA RNA-binding domain.
Cerrudo et al. (2014)
3 other articles

1 mention without citation

Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine.
Pioszak et al. (2005)