› 2cxx

X-ray diffraction
1.7Å resolution

Crystal structure of a probable GTP-binding protein engB

Released:
DOI: 10.2210/pdb2cxx/pdb
PDB entry 2cxx coloured by chain, front view.
PDB entry 2cxx coloured by chain, side view.
PDB entry 2cxx coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Kukimoto-Niino M, Murayama K, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable GTP-binding protein EngB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 190 amino acids
Theoretical weight: 22.25 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57939 (Residues: 27-216; Coverage: 88%)
Gene names: PH0200, engB
Sequence domains: 50S ribosome-binding GTPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 3 x GDP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P31
Unit cell:
a: 75.357Å b: 75.357Å c: 79.112Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 0.202
Expression system: Escherichia coli

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