› 2cz5

X-ray diffraction
1.85Å resolution

Crystal structure of orotidine 5'-phosphate decarboxylase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2cz5/pdb
PDB entry 2cz5 coloured by chain, front view.
PDB entry 2cz5 coloured by chain, side view.
PDB entry 2cz5 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Arai R, Ito K, Kamo-Uchikubo T, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 22.72 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58462 (Residues: 1-208; Coverage: 100%)
Gene names: PH0731, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 44.427Å b: 59.909Å c: 152.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.231
Expression system: Escherichia coli

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