2czj

X-ray diffraction
3.01Å resolution

Crystal structure of the tRNA domain of tmRNA from Thermus thermophilus HB8

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-186031 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
SsrA-binding protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 123 amino acids
Theoretical weight: 14.18 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RR57 (Residues: 1-123; Coverage: 85%)
Gene names: TTHA0902, smpB
Sequence domains: SmpB protein
Structure domains: Small Protein B; Chain: A;
tmRNA (63-MER) Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 63 nucleotides
Theoretical weight: 20.29 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided
Sequence domains: transfer-messenger RNA

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 84.776Å b: 67.957Å c: 178.662Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.255 0.255 0.32
Expression systems:
  • Escherichia coli
  • Not provided