› 2czw

X-ray diffraction
1.9Å resolution

Crystal structure analysis of protein component Ph1496p of P.horikoshii ribonuclease P

Released:
DOI: 10.2210/pdb2czw/pdb
PDB entry 2czw coloured by chain, front view.
PDB entry 2czw coloured by chain, side view.
PDB entry 2czw coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3.
Fukuhara H, Kifusa M, Watanabe M, Terada A, Honda T, Numata T, Kakuta Y, Kimura M
Biochem Biophys Res Commun 343 956-64 (2006)
PMID: 16574071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein eL8 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.57 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: P62009 (Residues: 1-123; Coverage: 100%)
Gene names: PH1496, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P21212
Unit cell:
a: 44.561Å b: 82.94Å c: 32.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.238
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Unexpected diversity of RNase P, an ancient tRNA processing enzyme: challenges and prospects.
Lai et al. (2010)
7 more

1 mention without citation

Of proteins and RNA: the RNase P/MRP family.
Esakova et al. (2010)