› 2d13

X-ray diffraction
2.4Å resolution

Crystal Structure of PH1257 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2d13/pdb
PDB entry 2d13 coloured by chain, front view.
PDB entry 2d13 coloured by chain, side view.
PDB entry 2d13 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Lokanath NK, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphthamide synthase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 227 amino acids
Theoretical weight: 25.81 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58996 (Residues: 1-227; Coverage: 100%)
Gene name: PH1257
Sequence domains: Diphthamide synthase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 55.974Å b: 96.696Å c: 84.921Å
α: 90° β: 93.77° γ: 90°
R-values:
R R work R free
0.229 0.229 0.25
Expression system: Escherichia coli

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