› 2d1t

X-ray diffraction
1.45Å resolution

Crystal structure of the thermostable Japanese Firefly Luciferase red-color emission S286N mutant complexed with High-energy intermediate analogue

Released:
DOI: 10.2210/pdb2d1t/pdb
PDB entry 2d1t coloured by chain, front view.
PDB entry 2d1t coloured by chain, side view.
PDB entry 2d1t coloured by chain, top view.
Source organism: Luciola cruciata
Primary publication:
Structural basis for the spectral difference in luciferase bioluminescence.
Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H
Nature 440 372-6 (2006)
PMID: 16541080

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 60.16 KDa
Source organism: Luciola cruciata
Expression system: Escherichia coli
UniProt:
  • Canonical: P13129 (Residues: 1-548; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SLU 1 x SLU
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 57.468Å b: 181.174Å c: 52.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.182
Expression system: Escherichia coli

Quick links

Citations

40 review citations

High-throughput screening assays for the identification of chemical probes.
Inglese et al. (2007)
39 more

4 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
3 more