› 2d27

X-ray diffraction
2.21Å resolution

Structure of the N-terminal domain of XpsE (crystal form I4122)

Released:
DOI: 10.2210/pdb2d27/pdb
PDB entry 2d27 coloured by chain, front view.
PDB entry 2d27 coloured by chain, side view.
PDB entry 2d27 coloured by chain, top view.
Source organism: Xanthomonas campestris
Primary publication:
Structure and function of the XpsE N-terminal domain, an essential component of the Xanthomonas campestris type II secretion system.
Chen Y, Shiue SJ, Huang CW, Chang JL, Chien YL, Hu NT, Chan NL
J Biol Chem 280 42356-63 (2005)
PMID: 16162504

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-152078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein E Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.96 KDa
Source organism: Xanthomonas campestris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31742 (Residues: 1-149; Coverage: 26%)
Gene names: XCC0660, pefE, xpsE
Sequence domains: MshEN domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: I4122
Unit cell:
a: 92.479Å b: 92.479Å c: 123.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Regulation and secretion of Xanthomonas virulence factors.
Büttner et al. (2010)
3 more

4 mentions without citation

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
3 more