› 2d3m

X-ray diffraction
1.6Å resolution

Pentaketide chromone synthase complexed with coenzyme A

Released:
DOI: 10.2210/pdb2d3m/pdb
PDB entry 2d3m coloured by chain, front view.
PDB entry 2d3m coloured by chain, side view.
PDB entry 2d3m coloured by chain, top view.
Source organism: Aloe arborescens
Primary publication:
Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens.
Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T
Chem Biol 14 359-69 (2007)
PMID: 17462571

Function and Biology Details

Reaction catalysed: 
5 malonyl-CoA = 5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,7-dihydroxy-2-methylchromone synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 44.87 KDa
Source organism: Aloe arborescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58VP7 (Residues: 1-403; Coverage: 100%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand COA 2 x COA
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P21
Unit cell:
a: 73.15Å b: 88.4Å c: 70.04Å
α: 90° β: 95.58° γ: 90°
R-values:
R R work R free
0.198 0.198 0.207
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The biosynthetic logic of polyketide diversity.
Hertweck C. (2009)
10 more

2 mentions without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)
1 more