› 2d45

X-ray diffraction
3.8Å resolution

Crystal structure of the MecI-mecA repressor-operator complex

Released:
DOI: 10.2210/pdb2d45/pdb
PDB entry 2d45 coloured by chain, front view.
PDB entry 2d45 coloured by chain, side view.
PDB entry 2d45 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec.
Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 320-4 (2006)
PMID: 16582476

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115155 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Methicillin resistance regulatory protein MecI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68261 (Residues: 1-123; Coverage: 100%)
Gene names: SA0040, mecI
Sequence domains: Penicillinase repressor
Structure domains:
5'-D(P*TP*AP*CP*TP*AP*CP*AP*TP*AP*TP*GP*TP*AP*GP*TP*A)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3212
Unit cell:
a: 61.464Å b: 61.464Å c: 419.777Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.264 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Mechanisms of Methicillin Resistance in Staphylococcus aureus.
Peacock et al. (2015)
2 more

5 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
4 more