› 2d5a

X-ray diffraction
1.7Å resolution

hypothetical protein from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2d5a/pdb
PDB entry 2d5a coloured by chain, front view.
PDB entry 2d5a coloured by chain, side view.
PDB entry 2d5a coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural basis of CoA recognition by the Pyrococcus single-domain CoA-binding proteins.
Hiyama TB, Zhao M, Kitago Y, Yao M, Sekine S, Terada T, Kuroishi C, Liu ZJ, Rose JP, Kuramitsu S, Shirouzu M, Watanabe N, Yokoyama S, Tanaka I, Wang BC
J Struct Funct Genomics 7 119-29 (2006)
PMID: 17342453

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129774 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CoA-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.75 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58836 (Residues: 1-144; Coverage: 100%)
Gene name: PH1109
Sequence domains: CoA binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6522
Unit cell:
a: 70.118Å b: 70.118Å c: 141.712Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

A de novo protein binding pair by computational design and directed evolution.
Karanicolas et al. (2011)