› 2d5i

X-ray diffraction
2.2Å resolution

The crystal structure of AzoR (Azo Reductase) from Escherichia coli

Released:
DOI: 10.2210/pdb2d5i/pdb
PDB entry 2d5i coloured by chain, front view.
PDB entry 2d5i coloured by chain, side view.
PDB entry 2d5i coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms.
Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M
J Biol Chem 281 20567-76 (2006)
PMID: 16684776

Function and Biology Details

Reaction catalysed: 
Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154392 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FMN-dependent NADH:quinone oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 21.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41407 (Residues: 2-201; Coverage: 100%)
Gene names: JW1409, acpD, azoR, b1412
Sequence domains: Flavodoxin-like fold
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P4212
Unit cell:
a: 94.63Å b: 94.63Å c: 54.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

The intestinal microbiota, gastrointestinal environment and colorectal cancer: a putative role for probiotics in prevention of colorectal cancer?
Azcárate-Peril et al. (2011)
4 more

1 mention without citation

Diverse but desolate landscape of gut microbial azoreductases: A rationale for idiopathic IBD drug response.
Simpson et al. (2023)