2d7d

X-ray diffraction
2.1Å resolution

Structural insights into the cryptic DNA dependent ATP-ase activity of UvrB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115159 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
UvrABC system protein B Chain: A
Molecule details ›
Chain: A
Length: 661 amino acids
Theoretical weight: 76.44 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37954 (Residues: 1-661; Coverage: 100%)
Gene names: BSU35170, dinA, uvr, uvrB
Sequence domains:
Structure domains:
UvrABC system protein B Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.69 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37954 (Residues: 622-661; Coverage: 6%)
Gene names: BSU35170, dinA, uvr, uvrB
Sequence domains: UvrB/uvrC motif
5'-D(P*TP*TP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 3 nucleotides
Theoretical weight: 868 Da

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 74.231Å b: 98.215Å c: 95.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.285
Expression system: Escherichia coli