2d7g

X-ray diffraction
3.3Å resolution

Crystal structure of the aa complex of the N-terminal domain of PriA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-115160 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Primosomal protein N' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17888 (Residues: 1-105; Coverage: 14%)
Gene names: JW3906, b3935, priA
Sequence domains: 3'DNA-binding domain (3'BD)
Structure domains: PriA, 3(prime) DNA-binding domain
DNA (5'-D(P*AP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 2 nucleotides
Theoretical weight: 581 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R32
Unit cell:
a: 110.82Å b: 110.82Å c: 267.183Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.26 0.309
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided