› 2d7u

X-ray diffraction
2.5Å resolution

Crystal structure of hypothetical adenylosuccinate synthetase, PH0438 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2d7u/pdb
PDB entry 2d7u coloured by chain, front view.
PDB entry 2d7u coloured by chain, side view.
PDB entry 2d7u coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Xie Y, Kishisita S, Murayama K, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-129678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 37.28 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58187 (Residues: 1-339; Coverage: 100%)
Gene names: PH0438, purA
Sequence domains: Adenylosuccinate synthetase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P3212
Unit cell:
a: 57.2Å b: 57.2Å c: 170.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.285
Expression system: Escherichia coli BL21(DE3)

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