› 2d8a

X-ray diffraction
2.05Å resolution

Crystal Structure of PH0655 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2d8a/pdb
PDB entry 2d8a coloured by chain, front view.
PDB entry 2d8a coloured by chain, side view.
PDB entry 2d8a coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-threonine + NAD(+) = L-2-amino-3-oxobutanoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-threonine 3-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 38.15 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58389 (Residues: 1-348; Coverage: 100%)
Gene names: PH0655, tdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand ZN 5 x ZN
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I222
Unit cell:
a: 85.084Å b: 89.436Å c: 122.413Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.235
Expression system: Escherichia coli

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