› 2dak

Solution NMR

Solution Structure of the Second UBA Domain in the Human Ubiquitin Specific Protease 5 (Isopeptidase 5)

Released:
DOI: 10.2210/pdb2dak/pdb
PDB entry 2dak coloured by chain, ensemble of 20 models, front view.
PDB entry 2dak coloured by chain, ensemble of 20 models, side view.
PDB entry 2dak coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Zhao C, Kigawa T, Sato M, Koshiba S, Inoue M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 5 Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 6.52 KDa
Source organism: Homo sapiens
Expression system: Cell free synthesis
UniProt:
  • Canonical: P45974 (Residues: 723-772; Coverage: 6%)
Gene names: ISOT, USP5
Sequence domains: UBA/TS-N domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, restrainted molecular dynamics
Expression system: Cell free synthesis

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