2dd8

X-ray diffraction
2.3Å resolution

Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-233308 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGG Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 25.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N355 (Residues: 20-234; Coverage: 98%)
Gene name: IGL@
Sequence domains:
Structure domains: Immunoglobulins
Spike protein S1 Chain: S
Molecule details ›
Chain: S
Length: 202 amino acids
Theoretical weight: 22.76 KDa
UniProt:
  • Canonical: P59594 (Residues: 317-518; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 64.693Å b: 68.611Å c: 80.085Å
α: 90° β: 98.08° γ: 90°
R-values:
R R work R free
0.202 0.199 0.261
Expression system: Escherichia coli