› 2ddh

X-ray diffraction
2.07Å resolution

Crystal Structure of Acyl-CoA oxidase complexed with 3-OH-dodecanoate

Released:
DOI: 10.2210/pdb2ddh/pdb
PDB entry 2ddh coloured by chain, front view.
PDB entry 2ddh coloured by chain, side view.
PDB entry 2ddh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Three-dimensional structure of rat-liver acyl-CoA oxidase in complex with a fatty acid: insights into substrate-recognition and reactivity toward molecular oxygen.
Tokuoka K, Nakajima Y, Hirotsu K, Miyahara I, Nishina Y, Shiga K, Tamaoki H, Setoyama C, Tojo H, Miura R
J Biochem 139 789-95 (2006)
PMID: 16672280

Function and Biology Details

Reaction catalysed: 
Acyl-CoA + O(2) = trans-2,3-dehydroacyl-CoA + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal acyl-coenzyme A oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 661 amino acids
Theoretical weight: 74.78 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07872 (Residues: 1-661; Coverage: 100%)
Gene names: Acox, Acox1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand HXD 1 x HXD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 85.5Å b: 103.2Å c: 161.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Flavoprotein oxidases: classification and applications.
Dijkman et al. (2013)
2 more

3 mentions without citation

Structural basis for substrate fatty acyl chain specificity: crystal structure of human very-long-chain acyl-CoA dehydrogenase.
McAndrew et al. (2008)
2 more