› 2de4

X-ray diffraction
1.8Å resolution

Crystal structure of DSZB C27S mutant in complex with biphenyl-2-sulfinic acid

Released:
DOI: 10.2210/pdb2de4/pdb
PDB entry 2de4 coloured by chain, front view.
PDB entry 2de4 coloured by chain, side view.
PDB entry 2de4 coloured by chain, top view.
Source organism: Rhodococcus sp. IGTS8
Primary publication:
Crystal structure and desulfurization mechanism of 2'-hydroxybiphenyl-2-sulfinic acid desulfinase.
Lee WC, Ohshiro T, Matsubara T, Izumi Y, Tanokura M
J Biol Chem 281 32534-9 (2006)
PMID: 16891315

Function and Biology Details

Reaction catalysed: 
2'-hydroxybiphenyl-2-sulfinate + H(2)O = 2-hydroxybiphenyl + sulfite
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2'-hydroxybiphenyl-2-sulfinate desulfinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 39.06 KDa
Source organism: Rhodococcus sp. IGTS8
Expression system: Escherichia coli
UniProt:
  • Canonical: P54997 (Residues: 1-365; Coverage: 100%)
Gene names: dszB, soxB
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand BPS 2 x BPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 153.37Å b: 45.86Å c: 112.9Å
α: 90° β: 115.93° γ: 90°
R-values:
R R work R free
0.169 0.169 0.199
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biodesulfurization of refractory organic sulfur compounds in fossil fuels.
Soleimani et al. (2007)
1 more

2 mentions without citation

Worldwide Protein Data Bank validation information: usage and trends.
Smart et al. (2018)
1 more