› 2de7

X-ray diffraction
2Å resolution

The substrate-bound complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

Released:
DOI: 10.2210/pdb2de7/pdb
PDB entry 2de7 coloured by chain, front view.
PDB entry 2de7 coloured by chain, side view.
PDB entry 2de7 coloured by chain, top view.
Source organisms:
Primary publication:
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system.
Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H
Structure 14 1779-89 (2006)
PMID: 17161368

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183053 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 44.91 KDa
Source organism: Janthinobacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84II6 (Residues: 1-384; Coverage: 100%)
Gene name: carAa
Sequence domains:
Structure domains:
Ferredoxin CarAc Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 115 amino acids
Theoretical weight: 12.45 KDa
Source organism: Pseudomonas resinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GI16 (Residues: 1-107; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

3 bound ligands:
Ligand FE2 3 x FE2
Ligand FES 6 x FES
Ligand 9CA 2 x 9CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 97.862Å b: 89.403Å c: 104.665Å
α: 90° β: 104.18° γ: 90°
R-values:
R R work R free
0.207 0.207 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Mononuclear non-heme iron enzymes with the 2-His-1-carboxylate facial triad: recent developments in enzymology and modeling studies.
Bruijnincx et al. (2008)
4 more

4 mentions without citation

Spectroscopic studies of the mononuclear non-heme Fe(II) enzyme FIH: second-sphere contributions to reactivity.
Light et al. (2013)
3 more