› 2dea

X-ray diffraction
1.24Å resolution

Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7

Released:
DOI: 10.2210/pdb2dea/pdb
PDB entry 2dea coloured by chain, front view.
PDB entry 2dea coloured by chain, side view.
PDB entry 2dea coloured by chain, top view.
Source organism: Vibrio proteolyticus
Primary publication:
The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica.
Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC
J Biol Inorg Chem 11 398-408 (2006)
PMID: 16596389

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial leucyl aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.24 KDa
Source organism: Vibrio proteolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01693 (Residues: 107-405; Coverage: 62%)
Sequence domains: Peptidase family M28
Structure domains: Zn peptidases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 109.605Å b: 109.605Å c: 90.816Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.153 0.163
Expression system: Escherichia coli

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Citations

2 review citations

Lysine biosynthesis in bacteria: a metallodesuccinylase as a potential antimicrobial target.
Gillner et al. (2013)
1 more

6 mentions without citation

Estrogenic activities of Fatty acids and a sterol isolated from royal jelly.
Suzuki et al. (2008)
5 more