› 2dei

X-ray diffraction
1.7Å resolution

Crystal Structure of galaktokinase from Pyrococcus horikoshii with AMP-PNP and galactose

Released:
DOI: 10.2210/pdb2dei/pdb
PDB entry 2dei coloured by chain, front view.
PDB entry 2dei coloured by chain, side view.
PDB entry 2dei coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Inagaki E, Sakamoto K, Shinkai A, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactokinase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.4 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58107 (Residues: 1-350; Coverage: 100%)
Gene names: PH0369, galK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLA 1 x GLA
Ligand MAP 1 x MAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 106.333Å b: 39.067Å c: 81.418Å
α: 90° β: 109.5° γ: 90°
R-values:
R R work R free
0.181 0.177 0.221
Expression system: Escherichia coli

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