› 2df8

X-ray diffraction
1.5Å resolution

Crystal Structure of the PH0510 protein from Pyrococcus horikoshii OT3 in complex with beta-D-Fructopyranose-1-phosphate

Released:
DOI: 10.2210/pdb2df8/pdb
PDB entry 2df8 coloured by chain, front view.
PDB entry 2df8 coloured by chain, side view.
PDB entry 2df8 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SIS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.86 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58246 (Residues: 1-325; Coverage: 100%)
Gene name: PH0510
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

2 bound ligands:
Ligand F1P 2 x F1P
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 61.179Å b: 79.644Å c: 67.494Å
α: 90° β: 105.24° γ: 90°
R-values:
R R work R free
0.182 0.181 0.191
Expression system: Escherichia coli

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