› 2dfa

X-ray diffraction
1.9Å resolution

Crystal Structure of Lactam Utilization Protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2dfa/pdb
PDB entry 2dfa coloured by chain, front view.
PDB entry 2dfa coloured by chain, side view.
PDB entry 2dfa coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-oxo-L-proline + 2 H(2)O = ADP + phosphate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-oxoprolinase subunit A Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 26.76 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG6 (Residues: 1-250; Coverage: 100%)
Gene names: TTHB195, pxpA
Sequence domains: LamB/YcsF family
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I222
Unit cell:
a: 63.781Å b: 63.826Å c: 124.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.232
Expression system: Escherichia coli BL21(DE3)

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