› 2dgl

X-ray diffraction
3.15Å resolution

Crystal structure of Escherichia coli GadB in complex with bromide

Released:
DOI: 10.2210/pdb2dgl/pdb
PDB entry 2dgl coloured by chain, front view.
PDB entry 2dgl coloured by chain, side view.
PDB entry 2dgl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB.
Gut H, Pennacchietti E, John RA, Bossa F, Capitani G, De Biase D, Grütter MG
EMBO J 25 2643-51 (2006)
PMID: 16675957

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase beta Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 466 amino acids
Theoretical weight: 52.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69910 (Residues: 1-466; Coverage: 100%)
Gene names: JW1488, b1493, gadB
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 6 x PLP
2 bound ligands:
Ligand BR 24 x BR
Ligand ACY 6 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6322
Unit cell:
a: 297.051Å b: 297.051Å c: 233.731Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.248
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Molecular aspects of bacterial pH sensing and homeostasis.
Krulwich et al. (2011)
9 more

1 mention without citation

Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB.
Gut et al. (2006)