› 2dkj

X-ray diffraction
1.15Å resolution

Crystal Structure of T.th.HB8 Serine Hydroxymethyltransferase

Released:
DOI: 10.2210/pdb2dkj/pdb
PDB entry 2dkj coloured by chain, front view.
PDB entry 2dkj coloured by chain, side view.
PDB entry 2dkj coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kai K, Goto M, Miyahara I, Hirotsu K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 44.68 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SI56 (Residues: 1-407; Coverage: 100%)
Gene names: TTHA1524, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 57.988Å b: 82.884Å c: 94.074Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.18 0.18 0.19
Expression system: Escherichia coli BL21(DE3)

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