› 2doo

X-ray diffraction
2.43Å resolution

The structure of IMP-1 complexed with the detecting reagent (DansylC4SH) by a fluorescent probe

Released:
DOI: 10.2210/pdb2doo/pdb
PDB entry 2doo coloured by chain, front view.
PDB entry 2doo coloured by chain, side view.
PDB entry 2doo coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Probing, inhibition, and crystallographic characterization of metallo-beta-lactamase (IMP-1) with fluorescent agents containing dansyl and thiol groups.
Kurosaki H, Yamaguchi Y, Yasuzawa H, Jin W, Yamagata Y, Arakawa Y
ChemMedChem 1 969-72 (2006)
PMID: 16937423

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.15 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52699 (Residues: 19-246; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand C4H 2 x C4H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 49.517Å b: 88.416Å c: 115.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Metallo-β-lactamase: inhibitors and reporter substrates.
Fast et al. (2013)
5 more

3 mentions without citation

Evolving carbapenemases: can medicinal chemists advance one step ahead of the coming storm?
Oelschlaeger et al. (2010)
2 more