› 2dp5

X-ray diffraction
3.55Å resolution

Structure of streptococcus pyogenes bacteriophage-associated hyaluronate lyase Hylp2

Released:
DOI: 10.2210/pdb2dp5/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 2dp5 coloured by chain, front view.
PDB entry 2dp5 coloured by chain, side view.
PDB entry 2dp5 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Entry authors: Mishra P, Bhakuni V, Prem Kumar R, Singh N, Sharma S, Kaur P, Singh TP

Function and Biology Details

Reaction catalysed: 
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major tropism determinant N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 35.83 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A0M7 (Residues: 7-337; Coverage: 98%)
Gene names: SPy_0701, hylP1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU300
Spacegroup: R32
Unit cell:
a: 59.587Å b: 59.587Å c: 588.546Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.236
Expression system: Escherichia coli

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