› 2dpd

X-ray diffraction
3.17Å resolution

Crystal structure of the Replication Termination Protein in complex with a pseudosymmetric B-site

Released:
DOI: 10.2210/pdb2dpd/pdb
PDB entry 2dpd coloured by chain, front view.
PDB entry 2dpd coloured by chain, side view.
PDB entry 2dpd coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Vivian JP, Wilce J, Wilce MCJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115179 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication termination protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 14.53 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CI76 (Residues: 1-122; Coverage: 100%)
Gene names: BSU18490, rtp
Sequence domains: Replication terminator protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*TP*AP*TP*GP*AP*AP*CP*AP*TP*AP*AP*TP*GP*TP*TP*CP*AP*TP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
5'-D(*CP*TP*AP*TP*GP*AP*AP*CP*AP*TP*TP*AP*TP*GP*TP*TP*CP*AP*TP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6422
Unit cell:
a: 117.006Å b: 117.006Å c: 165.553Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.236 0.278
Expression systems:
  • Escherichia coli BL21
  • Not provided

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