› 2dpn

X-ray diffraction
2.8Å resolution

Crystal Structure of the glycerol kinase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2dpn/pdb
PDB entry 2dpn coloured by chain, front view.
PDB entry 2dpn coloured by chain, side view.
PDB entry 2dpn coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites.
Sugahara M, Asada Y, Morikawa Y, Kageyama Y, Kunishima N
Acta Crystallogr D Biol Crystallogr 64 686-95 (2008)
PMID: 18560157

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 495 amino acids
Theoretical weight: 53.41 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53W24 (Residues: 1-495; Coverage: 100%)
Gene names: TTHB142, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 67.991Å b: 90.506Å c: 182.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

An overview of biological macromolecule crystallization.
Russo Krauss et al. (2013)
1 more

2 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
1 more