› 2dqb

X-ray diffraction
2.2Å resolution

Crystal structure of dNTP triphosphohydrolase from Thermus thermophilus HB8, which is homologous to dGTP triphosphohydrolase

Released:
DOI: 10.2210/pdb2dqb/pdb
PDB entry 2dqb coloured by chain, front view.
PDB entry 2dqb coloured by chain, side view.
PDB entry 2dqb coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure of dNTP-inducible dNTP triphosphohydrolase: insight into broad specificity for dNTPs and triphosphohydrolase-type hydrolysis.
Kondo N, Nakagawa N, Ebihara A, Chen L, Liu ZJ, Wang BC, Yokoyama S, Kuramitsu S, Masui R
Acta Crystallogr D Biol Crystallogr 63 230-9 (2007)
PMID: 17242516

Function and Biology Details

Reaction catalysed: 
dGTP + H(2)O = deoxyguanosine + triphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyguanosinetriphosphate triphosphohydrolase-like protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 376 amino acids
Theoretical weight: 42.9 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SL81 (Residues: 1-376; Coverage: 100%)
Gene name: TTHA0412
Sequence domains:
Structure domains: Hypothetical protein af1432

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P1
Unit cell:
a: 74.1Å b: 84.2Å c: 108Å
α: 110.2° β: 109.7° γ: 94.4°
R-values:
R R work R free
0.223 0.223 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

SAMHD1: Recurring roles in cell cycle, viral restriction, cancer, and innate immunity.
Mauney et al. (2018)
13 other articles

8 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
7 more