2dr0

X-ray diffraction
3.2Å resolution

Crystal structure of human carboxylesterase in complex with taurocholate

Released:

Function and Biology Details

Reactions catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
A steryl ester + H(2)O = a sterol + a fatty acid
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
PDBe Complex ID:
PDB-CPX-149882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Liver carboxylesterase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 542 amino acids
Theoretical weight: 59.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23141 (Residues: 19-561; Coverage: 99%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 55.419Å b: 179.954Å c: 201.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.255
Expression system: Spodoptera frugiperda