› 2dr6

X-ray diffraction
3.3Å resolution

Crystal structure of a multidrug transporter reveal a functionally rotating mechanism

Released:
DOI: 10.2210/pdb2dr6/pdb
PDB entry 2dr6 coloured by chain, front view.
PDB entry 2dr6 coloured by chain, side view.
PDB entry 2dr6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of a multidrug transporter reveal a functionally rotating mechanism.
Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A
Nature 443 173-9 (2006)
PMID: 16915237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1053 amino acids
Theoretical weight: 114.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DM2 1 x DM2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 227.046Å b: 134.561Å c: 161.697Å
α: 90° β: 98.08° γ: 90°
R-values:
R R work R free
0.302 0.298 0.359
Expression system: Escherichia coli K-12

Quick links

Citations

93 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
92 more

11 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
10 more