› 2dtj

X-ray diffraction
1.58Å resolution

Crystal structure of regulatory subunit of aspartate kinase from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb2dtj/pdb
PDB entry 2dtj coloured by chain, front view.
PDB entry 2dtj coloured by chain, side view.
PDB entry 2dtj coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Structural Insight into concerted inhibition of alpha 2 beta 2-type aspartate kinase from Corynebacterium glutamicum.
Yoshida A, Tomita T, Kurihara T, Fushinobu S, Kuzuyama T, Nishiyama M
J Mol Biol 368 521-36 (2007)
PMID: 17350037

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-150762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 19.38 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P26512 (Residues: 250-421; Coverage: 41%)
Gene names: Cgl0251, cg0306, lysC
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand THR 2 x THR
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 79.989Å b: 59.01Å c: 75.782Å
α: 90° β: 116.25° γ: 90°
R-values:
R R work R free
0.195 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Amino acid biosynthesis: new architectures in allosteric enzymes.
Curien et al. (2008)
2 more

4 mentions without citation

Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.
Lo et al. (2009)
3 more