› 2du7

X-ray diffraction
3.6Å resolution

Crystal structure of Methanococcus jannacshii O-phosphoseryl-tRNA synthetase

Released:
DOI: 10.2210/pdb2du7/pdb
PDB entry 2du7 coloured by chain, front view.
PDB entry 2du7 coloured by chain, side view.
PDB entry 2du7 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
Fukunaga R, Yokoyama S
Nat Struct Mol Biol 14 272-9 (2007)
PMID: 17351629

Function and Biology Details

Reaction catalysed: 
ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L-seryl-tRNA(Cys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-176772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-phosphoserine--tRNA(Cys) ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 549 amino acids
Theoretical weight: 63.53 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59054 (Residues: 1-539; Coverage: 100%)
Gene names: MJ1660, sepS
Sequence domains:
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 196.354Å b: 299.455Å c: 125.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.33 0.33 0.387
Expression system: Escherichia coli

Quick links

Citations

7 review citations

From one amino acid to another: tRNA-dependent amino acid biosynthesis.
Sheppard et al. (2008)
6 more

3 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
2 more