› 2dvm

X-ray diffraction
1.6Å resolution

NAD complex structure of PH1275 protein from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb2dvm/pdb
PDB entry 2dvm coloured by chain, front view.
PDB entry 2dvm coloured by chain, side view.
PDB entry 2dvm coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Lokanath NK, Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
439aa long hypothetical malate oxidoreductase (NAD) [malic enzyme] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 48.06 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59029 (Residues: 1-439; Coverage: 100%)
Gene name: PH1275
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand MES 3 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 101.856Å b: 74.294Å c: 141.157Å
α: 90° β: 100.37° γ: 90°
R-values:
R R work R free
0.191 0.191 0.207
Expression system: Escherichia coli

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