› 2dvr

X-ray diffraction
2.3Å resolution

Crystal structure analysis of the N-terminal bromodomain of human BRD2 complexed with acetylated histone H4 peptide

Released:
DOI: 10.2210/pdb2dvr/pdb
PDB entry 2dvr coloured by chain, front view.
PDB entry 2dvr coloured by chain, side view.
PDB entry 2dvr coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for acetylated histone H4 recognition by the human BRD2 bromodomain.
Umehara T, Nakamura Y, Jang MK, Nakano K, Tanaka A, Ozato K, Padmanabhan B, Yokoyama S
J Biol Chem 285 7610-8 (2010)
PMID: 20048151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-135463 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain-containing protein 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 122 amino acids
Theoretical weight: 14.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25440 (Residues: 73-194; Coverage: 15%)
Gene names: BRD2, KIAA9001, RING3
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H4 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 15 amino acids
Theoretical weight: 1.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P02309 (Residues: 2-16; Coverage: 15%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 21 x MSE
Ligand ALY 4 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 113.533Å b: 55.238Å c: 67.735Å
α: 90° β: 93.92° γ: 90°
R-values:
R R work R free
0.182 0.18 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

BET domain co-regulators in obesity, inflammation and cancer.
Belkina et al. (2012)
17 more

3 mentions without citation

Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
Patel et al. (2018)
2 more