2dwd

X-ray diffraction
2.6Å resolution

crystal structure of KcsA-FAB-TBA complex in Tl+

Released:
Primary publication:
Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel.
J Mol Biol 366 806-14 (2007)
PMID: 17196615

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-233231 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 10.98 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: I4
Unit cell:
a: 153.18Å b: 153.18Å c: 75.483Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.219 0.263
Expression system: Escherichia coli