› 2dwu

X-ray diffraction
1.6Å resolution

Crystal Structure of Glutamate Racemase Isoform RacE1 from Bacillus anthracis

Released:
DOI: 10.2210/pdb2dwu/pdb
PDB entry 2dwu coloured by chain, front view.
PDB entry 2dwu coloured by chain, side view.
PDB entry 2dwu coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.
May M, Mehboob S, Mulhearn DC, Wang Z, Yu H, Thatcher GR, Santarsiero BD, Johnson ME, Mesecar AD
J Mol Biol 371 1219-37 (2007)
PMID: 17610893

Function and Biology Details

Reaction catalysed: 
L-glutamate = D-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate racemase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 276 amino acids
Theoretical weight: 30.16 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6L8PVU3 (Residues: 1-276; Coverage: 100%)
Gene names: GBAA_0847, murI, racE1
Sequence domains: Asp/Glu/Hydantoin racemase
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand K 3 x K
Ligand DGL 3 x DGL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 85.783Å b: 49.659Å c: 198.252Å
α: 90° β: 90.065° γ: 90°
R-values:
R R work R free
0.162 0.162 0.194
Expression system: Escherichia coli BL21

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Citations

6 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
5 more

3 mentions without citation

Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.
May et al. (2007)
2 more