› 2dyj

X-ray diffraction
1.84Å resolution

Crystal structure of ribosome-binding factor A from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2dyj/pdb
PDB entry 2dyj coloured by chain, front view.
PDB entry 2dyj coloured by chain, side view.
PDB entry 2dyj coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural aspects of RbfA action during small ribosomal subunit assembly.
Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK
Mol Cell 28 434-45 (2007)
PMID: 17996707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-binding factor A Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.88 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJV1 (Residues: 1-95; Coverage: 100%)
Gene names: TTHA0907, rbfA
Sequence domains: Ribosome-binding factor A
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 28.426Å b: 65.838Å c: 43.173Å
α: 90° β: 96.18° γ: 90°
R-values:
R R work R free
0.199 0.199 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The universally conserved prokaryotic GTPases.
Verstraeten et al. (2011)
12 more

2 mentions without citation

Structural aspects of RbfA action during small ribosomal subunit assembly.
Datta et al. (2007)
1 more