› 2dze

X-ray diffraction
1.8Å resolution

Crystal structure of histone chaperone Asf1 in complex with a C-terminus of histone H3

Released:
DOI: 10.2210/pdb2dze/pdb
PDB entry 2dze coloured by chain, front view.
PDB entry 2dze coloured by chain, side view.
PDB entry 2dze coloured by chain, top view.
Source organisms:
Entry authors: Padmanabhan B, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130782 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone chaperone cia1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 18.35 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74515 (Residues: 1-161; Coverage: 62%)
Gene names: SPCC663.05c, asf1, cia1
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like
Histone H3.1/H3.2 Chain: X
Molecule details ›
Chain: X
Length: 10 amino acids
Theoretical weight: 1.29 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Not provided
UniProt:
  • Canonical: P09988 (Residues: 123-132; Coverage: 7%)
Gene names: SPAC1834.04, SPBC8D2.04, hht1, hht2, pi060

Ligands and Environments

1 bound ligand:
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 40.155Å b: 52.13Å c: 60.654Å
α: 107.35° β: 105.94° γ: 93.95°
R-values:
R R work R free
0.215 0.214 0.244
Expression systems:
  • Escherichia coli
  • Not provided

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