› 2e0c

X-ray diffraction
2Å resolution

crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain7 at 2.0 A resolution

Released:
DOI: 10.2210/pdb2e0c/pdb
PDB entry 2e0c coloured by chain, front view.
PDB entry 2e0c coloured by chain, side view.
PDB entry 2e0c coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
Crystal Structures of the Putative Isocitrate Dehydrogenase from Sulfolobus tokodaii Strain 7 in the Apo and NADP+-Bound Forms.
Kondo H, Murakami M
Archaea 2018 7571984 (2018)
PMID: 30662370

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
isocitrate dehydrogenase (NADP(+)) Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 46.56 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96YK6 (Residues: 1-409; Coverage: 100%)
Gene names: ST2166, STK_21660, idh
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 74.88Å b: 87.72Å c: 75.72Å
α: 90° β: 91.37° γ: 90°
R-values:
R R work R free
0.221 0.221 0.247
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Biochemical Characterization of Recombinant Isocitrate Dehydrogenase and Its Putative Role in the Physiology of an Acidophilic Micrarchaeon.
Winkler et al. (2021)

1 mention without citation

Crystal Structures of the Putative Isocitrate Dehydrogenase from Sulfolobus tokodaii Strain 7 in the Apo and NADP+-Bound Forms.
Kondo et al. (2018)