› 2e18

X-ray diffraction
2.1Å resolution

Crystal structure of project PH0182 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2e18/pdb
PDB entry 2e18 coloured by chain, front view.
PDB entry 2e18 coloured by chain, side view.
PDB entry 2e18 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Shimizu K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.9 KDa
Source organism: Pyrococcus horikoshii OT3
UniProt:
  • Canonical: O57921 (Residues: 1-257; Coverage: 100%)
Gene names: PH0182, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand IMD 3 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41
Unit cell:
a: 84.919Å b: 84.919Å c: 113.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.262

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