› 2e27

X-ray diffraction
1.7Å resolution

Crystal structure of Fv fragment of anti-ciguatoxin antibody complexed with ABC-ring of ciguatoxin

Released:
DOI: 10.2210/pdb2e27/pdb
PDB entry 2e27 coloured by chain, front view.
PDB entry 2e27 coloured by chain, side view.
PDB entry 2e27 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Critical contribution of aromatic rings to specific recognition of polyether rings. The case of ciguatoxin CTX3C-ABC and its specific antibody 1C49.
Tsumoto K, Yokota A, Tanaka Y, Ui M, Tsumuraya T, Fujii I, Kumagai I, Nagumo Y, Oguri H, Inoue M, Hirama M
J Biol Chem 283 12259-66 (2008)
PMID: 18326040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233710 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-ciguatoxin antibody, light chain Chain: L
Molecule details ›
Chain: L
Length: 119 amino acids
Theoretical weight: 13.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
anti-ciguatoxin antibody, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 126 amino acids
Theoretical weight: 13.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand DMS 2 x DMS
Ligand GOL 5 x GOL
Ligand AB0 1 x AB0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I212121
Unit cell:
a: 47.219Å b: 97.502Å c: 115.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rich et al. (2010)
6 more

5 mentions without citation

Identification of the viridicatumtoxin and griseofulvin gene clusters from Penicillium aethiopicum.
Chooi et al. (2010)
4 more