› 2e30

Solution NMR

Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1

Released:
DOI: 10.2210/pdb2e30/pdb
PDB entry 2e30 coloured by chain, ensemble of 20 models, front view.
PDB entry 2e30 coloured by chain, ensemble of 20 models, side view.
PDB entry 2e30 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1.
Mishima M, Wakabayashi S, Kojima C
J Biol Chem 282 2741-51 (2007)
PMID: 17050540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148674 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcineurin B homologous protein 1 Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99653 (Residues: 1-195; Coverage: 100%)
Gene names: CHP, CHP1
Sequence domains: EF hand
Structure domains: EF-hand
Sodium/hydrogen exchanger 1 Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 5.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19634 (Residues: 503-545; Coverage: 5%)
Gene names: APNH1, NHE1, SLC9A1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

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Citations

9 review citations

Intracellular pH regulation in heart.
Vaughan-Jones et al. (2009)
8 more

4 mentions without citation

Comparative structural analysis of lipid binding START domains.
Thorsell et al. (2011)
3 more