› 2e32

X-ray diffraction
3.52Å resolution

Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase

Released:
DOI: 10.2210/pdb2e32/pdb
PDB entry 2e32 coloured by chain, front view.
PDB entry 2e32 coloured by chain, side view.
PDB entry 2e32 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the selection of glycosylated substrates by SCF(Fbs1) ubiquitin ligase.
Mizushima T, Yoshida Y, Kumanomidou T, Hasegawa Y, Suzuki A, Yamane T, Tanaka K
Proc Natl Acad Sci U S A 104 5777-81 (2007)
PMID: 17389369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159043 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
F-box only protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 297 amino acids
Theoretical weight: 33.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80UW2 (Residues: 1-297; Coverage: 100%)
Gene names: Fbs1, Fbx2, Fbxo2
Sequence domains:
Structure domains:
S-phase kinase-associated protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 166 amino acids
Theoretical weight: 18.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 100%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 65.942Å b: 109.816Å c: 153.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.299
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Diversity of degradation signals in the ubiquitin-proteasome system.
Ravid et al. (2008)
14 more

3 mentions without citation

Structural basis for the selection of glycosylated substrates by SCF(Fbs1) ubiquitin ligase.
Mizushima et al. (2007)
2 more